Taxonomic and functional profiling of a multicomponent BUGS biopreparation.

The mobilization of cellulolytic microbiota from natural resources is a promising method for creating biofertilizers, as it allows for the selection of bacteria with a wide range of metabolic functions. BUGS is an example of such a complex microbial consortium with a specific microbiota that was initially isolated from the soil, but subsequently radically diverged from it. The combination of traditional microbiological methods and modern sequencing technologies, using the example of determining cellulolytic activity and network analysis, made it possible to determine the maceration phase of BUGS formed after the fourth month of composting. The active microbial BUGS community, according to the mag analysis, included both potentially purely cellulolytic microorganisms (for example, Steroidobacteraceae, Tepidisphaeraceae with a high pool content or Polyangiaceae with a high content of AA family genes) and the accompanying microbiota associated with nitrogen and sulfur metabolism. In addition, microorganisms unrelated to any of these functions have been associated with the active phase (for example, the potential cellulolytic Chryseolinea and its putative symbiont Gracilibacteria with unknown function). Using the example of BUGS, we have demonstrated that in complex biofertilizers, microorganisms associated with the decomposition of cellulose can perform various functions, the role of which has yet to be determined.
This study was supported by the Russian Science Foundation 23-16-00147.
The amplicon libraries of the 16C rRNA gene were sequenced on the Miseq Illumina platform, USA, and complete metagenomes on the Oxford nanopore Minion platform were performed on the basis of the Genomic Technologies, Proteomics, and Cell Biology Center of the Federal State Budgetary Budgetary Institution ARRIAM.
Gladkov G.V., Kimeklis A.K., Orlova O.V., Lisina T.O., Aksenova T.S., Kichko A.A., Pinaev A.G., Andronov E.E. Taxonomic and metagenomic review of biofertilizers based on peat decomposing straw. Microorganisms 2025, 13, 2830. https://doi.org/10.3390/microorganisms13122830