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Laboratory of signal regulation №5

Laboratory of signal regulation №5

Head of the laboratory : Долгих Елена Анатольевна

Telephone: +7 (812) 476 24 96

The laboratory of signal regulation was established in 2019 as part of the implementation of the national project "Science and Universities". The laboratory consists of 12 employees. The head of the laboratory is Elena A. Dolgikh, Doctor of Biological Sciences, laureate of the prize of the Government of the Russian Federation in the field of science and technology "Production of chitin derivatives and preparations based on them for use in agriculture, medicine, food industry and biotechnology" (2013).

The main purpose of the laboratory is to study the signaling exchange between plants and microorganisms, aimed at improving the technology of obtaining and using signaling compounds as effective stimulators of plant development, which are of important agricultural importance. Among such compounds are chitin derivatives, which are secreted by microorganisms and used as stimulants for the development of mutualistic symbioses and effective means of biological protection in plants.

 

Основные результаты работы

Основные направления

  • Search and study of plant receptors for signaling molecules (Nod factors, chitooligosaccharides, Myc factors) secreted by nodule bacteria and fungi of arbuscular mycorrhiza

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  • Identification and analysis of components of signaling pathways that are activated by receptors using transcriptomic and proteomic analysis methods

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  • To study the effect of genes encoding receptors for Nod factors and controlling the initiation of the development of nitrogen-fixing symbioses on non-leguminous plants in order to assess the possibility of interaction of such plants with nitrogen-fixing b

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  • To study the role of hormones in the control of plant-microbial relationships and to clarify how regulators of hormonal pathways interact with transcription factors activated by signaling molecules

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Staff

Participation in projects:

  1. ОНТП задание (№ FGEW -2021-0005).
  2. РНФ 21-16-00106 «To study the immunosuppressive properties of signaling molecules of nodule bacteria and fungi of arbuscular mycorrhiza, ensuring the development of effective intracellular symbioses.» (2021-2023; head: Dolgikh E.A., participants: Leppyanen I.V., Pavlova O.A., Rudaya E.S., Bovin A.D., Dymov A.M., Dolgikh A.V., Vikhnina M.A.).
  3. РНФ 22-26-00279 «Search for targets of key transcription factors IPD3/CYCLOPS and NIN regulating the process of nodule formation in seed peas" (2021-2022; supervisor: Dolgikh A.V., participants: Rudaya E.S., Dymo A.M., Shirobokova S.A.).
  4. НЦМУ «Agrotechnologies of the future" (2021-2025; Head of the topic – Dolgikh E.A., participants: Bovin A.D., Rudaya E.S., Dolgikh A.V.).

 

Participation in international conferences in 2019 - 2021:

  1. Participation in the 14th European Nitrogen Fixation Conference - 14th European nitrogen fixation conference, 22 September – 22 October 2021. Aarhus, Denmark. (2 invited presentations and 2 poster presentations).
  2. Participation in the European Biotechnology Conference - European Biotechnology Congress 2020. Prague, September 24-26, 2020. (2 invited presentations).
  3. Participation in the 9th International Conference on Legume Genetics and Genomics - the 9th International Conference on Legume Genetics and Genomics. Dijon, France, 13-17 May 2019 (1 invited presentation).

List of publications 2019-2022:

  1. Bovin A.D., Pavlova O.A., Dolgikh A.V., Leppyanen I.V., Dolgikh E.A. The role of heterotrimeric G-protein beta subunits during nodulation in Medicago truncatula Gaertn and Pisum sativum L. // Frontiers in Plant Science. 2022. V. 12: Article 808573. doi.org/10.3389/fpls.2021.808573 (WoS Q1, Scopus).
  2. Rudaya E.S., Kozulina P.Yu., Pavlova O.A., Dolgikh A.V., Ivanova A.N., Dolgikh E.A.  Regulation of the later stages of nodulation stimulated by IPD3/CYCLOPS transcription factor and cytokinin in pea Pisum sativum L. // Plants. 2022. V. 11 (1). Article 56. doi.org/10.3390/plants11010056 (WoS Q1, Scopus)
  3. Leppyanen I.V., Pavlova O.A., Vashurina M.A., Bovin A.D., Dolgikh A.V., Shtark O.Y., Sendersky I. V., Dolgikh V.V., Tikhonovich I.A., Dolgikh E.A. LysM-receptor-like kinase LYK9 of Pisum sativum L. may regulate plant responses to chitooligosaccharides differing in structure // Int. J. Mol. Science. 2021. V. 22 (2). Article 711. doi.org/10.3390/ijms22020711 (WoS Q1, Scopus).
  4. Pavlova O.А., Leppyanen I.V., Kustova D.V., Bovin A. D., Dolgikh E. A. Phylogenetic and structural analysis of annexins in pea (Pisum sativum L.) and their role in legume–rhizobial symbiosis development // Vavilov Journal of Genetics and Breeding. 2021. V. 25, N 5. P. 502-513. doi: 10.18699/VJ21.057 (WoS Q4, Scopus).
  5. Sergeeva AV, Belashova TA, Bondarev SA, Velizhanina M.E., Barbitoff YA, Matveenko AG, Valina AA, Simanova AL, Zhouravleva GA, Galkin AP. Direct proof of the amyloid nature of yeast prions [PSI+] and [PIN+] by the method of immunoprecipitation of native fibrils // FEMS Yeast Res. 2021. V. 21(6). Article foab046. doi: 10.1093/femsyr/foab046 (WoS Q1, Scopus).
  6. Chirinskaite AV, Siniukova VA, Velizhanina M.E., Sopova JV, Belashova TA, Zadorsky SP. STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. Prion. 2021. 15(1): 29-36. doi: 10.1080/19336896.2021.1883980 (WoS Q2, Scopus).
  7. Pavlova O. A., Kirienko A. N., Sendersky I. V., Dolgikh V. V. and Dolgikh E. A. Heterologous synthesis of plant LysM receptor-like kinase K1 in insect cells and binding assay with ligand // Biotechnology & Biotechnological Equipment. 2021. V. 35: sup1, S94. doi: 10.1080/13102818.2020.1871545 (WoS Q4, Scopus).
  8. Pavlova O. A., Leppyanen I. V., Senderskiy I. V., Dolgikh V. V., Tikhonovich I. A. and Dolgikh E. A. Synthesis of plant receptor-like kinases involved in interaction with pathogenic and beneficial fungi in heterologous system and their functional analysis // Biotechnology & Biotechnological Equipment. 2021. V. 35: sup1, S92. doi: 10.1080/13102818.2020.1871545 (WoS Q4, Scopus).
  9. Rudaya E.S., Dolgikh E.A. Preparation and analysis of composite tomato plants Solanum lycopersicum L. transformed by genes of pea receptors to rhizobium signaling molecules. Agricultural biology. 2021. Vol. 56, No. 3, pp. 465-474. doi: 10.15389/agrobiology.2021.3.465rus (WoS Q3, Scopus, RSCI).
  10. Dolgikh A.V., Dolgikh E.A. Search for regulators interacting with the transcription factor BEL1 and necessary to control the development of legume-rhizobial symbiosis. Ecological genetics. 2021. Vol. 19, No. 1, pp. 37-45. doi: https://doi.org/10.17816/ecogen51489 (Was Q3, Scopus, RSCI).
  11. Leppianen I.V., Stark O.Yu., Pavlova O.A., Bovin A.D., Ivanova K.A., Serova T.S., Dolgikh E.A. Analysis of the effects of joint inoculation by arbuscular mycorrhiza fungi and rhizobia on the growth and development of pea plants Pisum sativum L. Agricultural biology. 2021. Vol. 56, No. 3, pp. 475-486 doi: 10.15389/agrobiology.2021.3.475rus (WoS Q3, Scopus, RSCI).
  12. Dolgikh A.V., Rudaya E.S., Dolgikh E.A. Identification of BELL transcription factors involved in nodule initiation and development in the legumes Pisum sativum and Medicago truncatula. Plants. 2020. V. 9 (12), 1808. doi:10.3390/plants9121808 (WoS Q1, Scopus, РИНЦ).
  13. Dolgikh E.A., Kusakin P.G., Kitaeva A.B., Tsyganova A.V., Kirienko A.N., Leppyanen I.V., Dolgikh A.V., Ilina E.L., Demchenko K.N., Tikhonovich I.A., Tsyganov V. E. Mutational analysis indicates that abnormalities in rhizobial infection and subsequent plant cell and bacteroid differentiation in pea (Pisum sativum) nodules coincide with abnormal cytokinin responses and localization. Annals of Botany, 2020. 125: 905–923.  doi: 10.1093/aob/mcaa022 (WoS Q1, Scopus, РИНЦ).
  14. Smolikova G., Shiroglazova O., Vinogradova G., Leppyanen I., Dinastiya E., Yakovleva O., Dolgikh E., Titova G., Frolov A., Medvedev S. Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars. Funct Plant Biol. 2020. 47(5): 409-424. doi: 10.1071/FP19270 (WoS Q1, Scopus, РИНЦ).
  15. Afonin A.M., Leppyanen I.V., Kulaeva O.A., Shtark O.Y., Tikhonovich I.A., Dolgikh E.A., Zhukov V.A. A high coverage reference transcriptome assembly of pea (Pisum sativum L.) mycorrhizal roots. Vavilov Journal of Genetics and Breeding 2020. 24 (4): 331-339  doi:10.18699/VJ20.625 (WoS Q4, Scopus, РИНЦ).
  16. Bovin A. D., Leppyanen I. V., Pavlova O. A., Dolgikh E. A. The role of heterotrimeric G proteins in the control of symbiosis development in legume plants. BIO Web of Conferences, 2020, 23: 03004 doi.org/10.1051/bioconf/20202303004 (WoS Q4, Scopus, РИНЦ).
  17. Kirienko A.N., Dolgikh E. A. Studying the effect of tissue-specific expression of the K1 gene encoding LysM-receptor-like kinase on the development of symbiosis in peas. BIO Web of Conferences, 2020, 23: 03005 doi.org/10.1051/bioconf/20202303005 (WoS Q4, Scopus, РИНЦ).
  18. Kustova D.V., Dolgikh E.A. Annexins and their role in controlling the development of symbioses in plants. Ecological genetics. 2020. 18(3):293-300 doi.org/10.17816/ecogen.183 (Scopus).
  19. Lubyaga Y., Dolgikh A., Drozdova P., Nazarova A., Timofeyev M. Transcriptome- based analysis of the diversity of membrane-bound lectins in Baikal amphipods Eulimnogammarus sp. and the Holarctic amphipod Gammarus lacustris. Limnology and Freshwater Biology. 2020. 4 (SI:7VBC): 797-798. doi:10.31951/2658-3518-2020-A-4-797 (РИНЦ).
  20. Dolgikh A.V., Kirienko A.N., Tikhonovich I.A., Foo E., Dolgikh E.A. The DELLA proteins influence the expression of cytokinin biosynthesis and response genes during nodulation. Front Plant Sci. 2019. 10: 432. doi: 10.3389/fpls.2019.00432 (WoS Q1, Scopus, РИНЦ).
  21. Leppyanen I.V., Kirienko A.N., Dolgikh E.A. Agrobacterium rhizogenes - mediated transformation of Pisum sativum L. roots. PeerJ 2019. 7:e6552 doi.org/10.7717/ peerj.6552 (WoS Q1, Scopus, РИНЦ).
  22. Kirienko A.N., Vishnevskaya N.A., Kitaeva A.B., Shtark O.Y., Kozyulina P.Y., Thompson R., Dalmais M., Bendahmane A., Tikhonovich I.A., Dolgikh E.A. Structural variations in LysM domains of LysM-RLK PsK1 may result in a different effect on pea-rhizobial symbiosis development. Int. J. Mol. Sci. 2019. V. 20 (7): 1624.  doi:10.3390/ijms20071624 (WoS Q1, Scopus, РИНЦ).
  23. Sopova J., Koshel E., Belashova T., Zadorsky S., Sergeeva A., Siniukova V., Shenfeld A., Velizhanina M., Volkov K., Nizhnikov A., Gaginskaya E., Galkin A. (2019). RNA-binding protein FXR1 is presented in rat brain in amyloid form. Nature Sci Rep., 2019. 9(1): 18983. doi: 10.1038/s41598-019-55528-6 (WoS Q1, Scopus, РИНЦ).
  24. Pendina A.A., Shilenkova Y.V., Talantova O.E., Efimova O.A., Chiryaeva O.G., Malysheva O.V., Dudkina V.S., Petrova L.I., Serebryakova E.A., Shabanova E.S., Mekina I.D., Komarova E.M., Koltsova A.S., Tikhonov A.V., Tral T.G., Tolibova G.K., Osinovskaya N.S., Krapivin M.I., Petrovskaia-Kaminskaia A.V., Korchak T.S., Ivashchenko T.E., Glotov O.S., Romanova O.V., Shikov A.E., Urazov S.P., Tsay V.V., Eismont Y.A., Scherbak S.G., Sagurova Y.M., Vashukova E.S., Kozyulina P.Y., Dvoynova N.M., Glotov A.S., Baranov V.S., Gzgzyan A.M., Kogan I.Y. Reproductive History of a Woman With 8p and 18p Genetic Imbalance and Minor Phenotypic Abnormalities // Front Genet. 2019 Nov 20;10:1164. doi: 10.3389/fgene.2019.01164. eCollection 2019 (WoS Q1, Scopus, РИНЦ).
  25. Bovin A.D., Dolgikh E.A. The role of heterotrimeric G-proteins in signaling regulation in plants // Ecological genetics. 2019. Vol. 17, No. 2. pp. 43-54 doi.org/10.17816/ecogen17243-54 (Scopus, RSCI).
  26. Senderskiy I. V., Tsarev A. A., Zhuravlyov V. S., Pavlova O. A., Dolgikh V. V. Heterologous expression of Nosema bombycis hexokinase in baculovirus-Sf9 insect cell system confirms its accumulation in host nuclei and secretion by the microsporidian parasite. Protistology  14 (1),  2019 (Scopus, РИНЦ).
  27. Dolgikh A.V., Dolgikh E. A. The role of universal regulators of plant growth and development of DELLA proteins in the control of symbioses // Ecological genetics.  2019. Т. 17,  № 1.  С. 33–41 (Scopus, РИНЦ).

 

Patents:

  1. Dolgikh E.A., Kiriyenko A.N. A recombinant vector for the synthesis of receptor kinase K1 in plant cells, which controls the development of symbiosis with nodule bacteria, and a strain for replication of the vector. Patent No. 2738735 dated 12.12.2018. The patent was registered on 12/16/2020.
  2. Dolgikh E.A., Dolgikh V.V., Leppyanen I.V., Varlamov V.P., Lopatin S.A., Tikhonovich I.A. Method of enzymatic production of penta-N-acetylchytopentaose // Patent No. 2460800 dated 08/04/2010. The patent was registered on 09/10/2012.
  3. Dolgikh E.A., Dolgikh V.V., Leppyanen I.V., Varlamov V.P., Lopatin S.A., Tikhonovich I.A. Method of enzymatic production of penta-N-acetylchitopentaose and hexa-N-acetylchitohexaose. Patent No. 2517620 dated 08/04/2010. The patent was registered on 04/01/2014.

 

Dissertations:

Bovin A.D. "The role of heterotrimeric G-proteins in the regulation of the development of legume-rhizobial symbiosis in alfalfa M. truncatula Gaertn. and peas P. sativum L." specialty 03.02.03 microbiology, 2022

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