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КThe team of the Laboratory for Proteomics of Supra-organismal Systems is engaged in the study of molecular mechanisms that determine pathogenic and functional properties of various bacteria; identification of new prion and amyloid proteins of prokaryotes and eukaryotes; detection of protein networks and regulatory cascades that control the species specificity in symbiotic relationships; development of multifunctional microbiological preparations, conducts fundamental and applied research on a number of other interdisciplinary fields. Thus, in recent years, in the Laboratory for the first time in world practice, amyloid proteins have been identified in plants [Antonets et al., PLOS Biology, 2020] and symbiotic bacteria [Kosolapova et al., Biomolecules, 2020], and effective and accurate bioinformatic program has been developed to predict the bacterial genes encoding Cry toxins, which are important for biotechnology [Shikov et al., Toxins, 2020]. The Laboratory uses a wide range of different approaches, including genomics, transcriptomics, proteomics, and bioinformatics; microbial genetics and microbiology; a number of molecular biological methods, including genetic engineering, quantitative analysis of gene expression, various methods of protein analysis, as well as microscopy (confocal, polarized light, electron). The laboratory has a rich history; it was established in 1891 as a "Bacteriological Laboratory at the Department of Agriculture and State Property"; academician of the USSR Academy of Sciences Б.Л. Исаченко and professors A.E. Feoktistov, S.S. Merezhkovsky were at the origins of its creation and development. The main research conducted in the Laboratory was aimed at identifying and characterizing rodenticidal and bactericidal microorganisms. From 1897 to the present, the culture of Salmonella enteritidis var. Issatschenko, also called the "Isachenko bacterium", is constantly maintained in the Laboratory and has a strictly selective pathogenic effect within the order of rodents. In 1969-2011, the laboratory was headed by professor Н.В. Кандыбин, under whose guidance biological preparations based on the insecticidal bacterium Bacillus thuringiensis were created and implemented in industry. The main areas of research in the Laboratory are:
Some of the recent results: (A-C) The metalloprotease domain of the Escherichia coli YghJ protein, which plays an important role in infectious diseases caused by enterotoxigenic strains of this bacterium, forms amyloids, that is, protein fibrils with an ordered structure. The results of the work can be found in the publication of Belousov et al., PLOS One, 2018. (D) Seed storage proteins in plants, which are one of the main components of the human diet, have potentially amyloidogenic properties. The results of this study can be found in the publication by Antonets and Nizhnikov, IJMS, 2017. This work was supported by the Russian Science Foundation (Grant No 17-16-01100). (E-F) Asparagine-glutamine-rich fragment of the Saccharomyces cerevisiae Gln3 protein interacts with other asparagine-glutamine-rich amyloids. The results of the study can be found in the publication by Antonets and co-authors, Biochemistry (Moscow), 2016. |
Doctor of Biological Sciences, Professor of the Russian Academy of Sciences, Head of Laboratory No. 7, Professor of St. Petersburg State University
Phone: +7(812)476-16-02
Belousov M.V., Kosolapova A.O., Fayoud H., Sulatsky M.I., Sulatskaya A.I., Romanenko M.N., Bobylev A.G., Antonets K.S., Nizhnikov A.A. OmpC and OmpF Outer Membrane Proteins of Escherichiacoli and Salmonellaenterica Form BonaFideAmyloids // Int. J. Mol. Sci., 2023, V.24, e15522. https://doi.org/10.3390/ijms242115522
Merkushova A.V., Shikov A.E., Nizhnikov A.A., Antonets K.S. For Someone, You Are the Whole World: Host-Specificity of Salmonella enterica // Int. J. Mol. Sci., 2023, V.24, e13670. https://doi.org/10.3390/ijms241813670
Shikov A.E., Savina I.A., Nizhnikov A.A., Antonets K.S. Recombination in Bacterial Genomes: Evolutionary Trends // Toxins, 2023, V.15, e568. https://doi.org/10.3390/toxins15090568
Shikov A.E., Merkushova A.V., Savina I.A., Nizhnikov A.A., Antonets K.S. The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome // Frontiers in Microbiology, 2023, V.14, e1211999. https://doi.org/10.3389/fmicb.2023.1211999
Sulatsky M.I., Belousov M.V., Kosolapova A.O., Mikhailova E.V., Romanenko M.N., Antonets K.S., Kuznetsova I.M., Turoverov K.K., Nizhnikov A.A., Sulatskaya A.I. Amyloid Fibrils of Pisum sativum L. Vicilin Inhibit Pathological Aggregation of Mammalian Proteins // Int. J. Mol. Sci., 2023, V.24, e12932. https://doi.org/10.3390/ijms241612932