FSBSI ARRIAM
Federal State Budget Scientific Institution
All-Russia Research Institute for Agricultural Microbiology
All-Russia Research Institute for Agricultural Microbiology
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Using transcriptomic and proteomic analysis methods, a number of new regulators of signaling pathways have been identified that are necessary for signal transmission in plant cells when interacting with nodule bacteria and fungi. Among them are heterotrimeric G protein, phospholipases C and D, calcium and phospholipid-binding proteins. For the genes encoding the identified regulators, knockout was performed using RNA interference or overexpression was performed. This made it possible for the first time to show, for example, the important role of the heterotrimeric G protein, as well as the calcium-binding protein annexin, in controlling the development of legume-rhizobial symbiosis.
The analysis of signaling pathways activated by the receptor-like kinase LYK9 using transcriptomic and proteomic profiling technologies for the first time allowed us to show the key role of the complex of mitogen-activated protein kinases (MAP kinases) in signal transmission during recognition by the plant of XOC4-5 and XOC6-8. Studies have shown that as a result of activation of different MAP kinase complexes, the plant can control either the development of symbiosis with AM fungi when recognizing XOC4-5, or activate the immune response when recognizing XOC6-8.
