MAINPAGE
INSTITUTE
ADMINISTRATION
ACADEMIC COUNCIL
DEPARTMENTS
LABORATORY №1
LABORATORY №2
LABORATORY №3
LABORATORY №4
LABORATORY №5
LABORATORY №6
LABORATORY №7
LABORATORY №8
LABORATORY №9
LABORATORY №10
Scientific and educational group
NEWS
HISTORY
FAMOUS MICROBIOLOGISTS
STAFF
PARTNERS
PHOTOGALLERY
VIDEOS
PRESS
SCIENCE
SCIENTIFIC ACTIVITY
SCIENCE AND UNIVERSITIES
AWCSC "Agrotechnologies of the future"
COLLECTIONS
Collection of cultures of agricultural microorganisms (ARCAM)
CONTACTS

Laboratory of signal regulation №5

Laboratory of signal regulation №5

Head of the laboratory : Долгих Елена Анатольевна

Telephone: +7 (812) 476 24 96

The laboratory of signal regulation was established in 2019 as part of the implementation of the national project "Science and Universities". The laboratory consists of 12 employees. The head of the laboratory is Elena A. Dolgikh, Doctor of Biological Sciences, laureate of the prize of the Government of the Russian Federation in the field of science and technology "Production of chitin derivatives and preparations based on them for use in agriculture, medicine, food industry and biotechnology" (2013).

The main purpose of the laboratory is to study the signaling exchange between plants and microorganisms, aimed at improving the technology of obtaining and using signaling compounds as effective stimulators of plant development, which are of important agricultural importance. Among such compounds are chitin derivatives, which are secreted by microorganisms and used as stimulants for the development of mutualistic symbioses and effective means of biological protection in plants.

 

Основные результаты работы

Основные направления

  • Search and study of plant receptors for signaling molecules (Nod factors, chitooligosaccharides, Myc factors) secreted by nodule bacteria and fungi of arbuscular mycorrhiza

    Узнать больше »

  • Identification and analysis of components of signaling pathways that are activated by receptors using transcriptomic and proteomic analysis methods

    Узнать больше »

  • To study the effect of genes encoding receptors for Nod factors and controlling the initiation of the development of nitrogen-fixing symbioses on non-leguminous plants in order to assess the possibility of interaction of such plants with nitrogen-fixing b

    Узнать больше »

  • To study the role of hormones in the control of plant-microbial relationships and to clarify how regulators of hormonal pathways interact with transcription factors activated by signaling molecules

    Узнать больше »

Staff

Participation in projects:

  1. ОНТП задание FGEW-2024-0006
  2. РНФ 24-16-00108 "The study of the effect of microRNAs, TLR receptors and effectors on the regulation of the immune response in legumes during intracellular symbiosis" (supervisor - Dolgikh E.A., main performers - Pavlova O.A., Kanzurova E.S., Bovin A.D.)
  3. ОНТП задание (№ FGEW -2021-0005).
  4. РНФ 21-16-00106 «To study the immunosuppressive properties of signaling molecules of nodule bacteria and fungi of arbuscular mycorrhiza, ensuring the development of effective intracellular symbioses.» (2021-2023; head: Dolgikh E.A., participants: Leppyanen I.V., Pavlova O.A., Rudaya E.S., Bovin A.D., Dymov A.M., Dolgikh A.V., Vikhnina M.A.).
  5. РНФ 22-26-00279 «Search for targets of key transcription factors IPD3/CYCLOPS and NIN regulating the process of nodule formation in seed peas" (2021-2022; supervisor: Dolgikh A.V., participants: Rudaya E.S., Dymo A.M., Shirobokova S.A.).
  6. НЦМУ «Agrotechnologies of the future" (2021-2025; Head of the topic – Dolgikh E.A., participants: Bovin A.D., Rudaya E.S., Dolgikh A.V.).

 

Participation in international conferences in 2019 - 2021:

  1. Participation in the 14th European Nitrogen Fixation Conference - 14th European nitrogen fixation conference, 22 September – 22 October 2021. Aarhus, Denmark. (2 invited presentations and 2 poster presentations).
  2. Participation in the European Biotechnology Conference - European Biotechnology Congress 2020. Prague, September 24-26, 2020. (2 invited presentations).
  3. Participation in the 9th International Conference on Legume Genetics and Genomics - the 9th International Conference on Legume Genetics and Genomics. Dijon, France, 13-17 May 2019 (1 invited paper).
  4. III International Conference "GMOs: history, achievements, social and environmental risks", St. Petersburg, October 3 - 5, 2023 – 2 invited reports
  5. VII International Scientific Conference, Kazan, Russia, July 10-15, 2023 - 4 invited papers
  6. Scientific Conference "Genomics, metagenomics and molecular Biology of microorganisms", Moscow, Skoltech, September 23-24, 2023 - 2 invited reports

List of publications 2019-2022:

  1. Dolgikh, A.V., Kantsurova, E.S., Dymo, A.M. et al. The Role of the BELL1-2 Transcription Factor in the Development of Legume-rhizobial Symbiosis. J Plant Growth Regul (2024). https://doi.org/10.1007/s00344-024-11487-5
  2. Bovin A.D., Dolgikh A.V., Dymo A.M., Kantsurova E.S., Pavlova O.A., Dolgikh E.A. Genetically modified legume plants as a basis for studying the signal regulation of symbiosis with nodule bacteria. Horticulturae. 2024. V. 10, 9. doi.org/10.3390/horticulturae10010009 (WoS Q1)
  3. Kantsurova E.S., Kozlov N.V., Dolgikh E.A. Development of approaches for genome editing of pea plants using CRISPR/Cas9 prime-editing technique. Ecological genetics. 2024. V. 22, N 1. P. 63-74. doi.org/10.17816/ecogen623140 (Scopus Q4).  
  4. Kozyulina P.Y., Pavlova O.A., Kantsurova (Rudaya) E.S., Bovin A.D., Shirobokova S.A., Dolgikh A.V., Dymo A.M., Dolgikh E.A. Transcriptomic analysis of pea plant responses to chitooligosaccharides' treatment revealed stimulation of mitogen-activated protein kinase cascade. Front Plant Sci. 2023. V. 14: 1092013. doi: 10.3389/fpls.2023.1092013. (WoS Q1).
  5. Dolgikh, A.V., Kantsurova, E.S. and Dolgikh, E.A. ChIP-Seq Analysis Protocol for Identification of PsIPD3 and PsNIN Transcription Factors Binding Sites in Pisum sativum Genome. Russian Journal of Plant Physiology, 2023. 70, 203. https://doi.org/10.1134/S1021443723603403 (WOS Q3).
  6. Dolgikh, A.V. and Dolgikh, E.A. RNA Sequencing Technologies at the Single Cell Level in Plants. Russian Journal of Plant Physiology, 2023. 70, 202. https://doi.org/10.1134/S1021443723602835 (WOS Q3).
  7. Kantsurova (Rudaya), E.S., Ivanova A.N., Kozyulina, P.Y., Dolgikh E.A. Exogenously applied cytokinin altered the bacterial release and subsequent stages of nodule development in pea ipd3/сyclops mutant. Plants. 2023, 12,657. https://doi.org/10.3390/plants12030657 (WoS Q1). 
  8. Gostev V, Sabinova K, Sopova J, Kalinogorskaya O, Sulian O, Chulkova P, Velizhanina M, Pavlova P, Danilov L, Kraeva L, Polev D, Martens E, Sidorenko S. Phenotypic and genomic characteristics of oxacillin-susceptible mecA-positive Staphylococcus aureus, rapid selection of high-level resistance to beta-lactams. Eur J Clin Microbiol Infect Dis. 2023. 42 (9): 1125-1133. doi: 10.1007/s10096-023-04646-1 (WoS Q1).
  9. Dolgikh E., Kantsurova E., Kozyulina P., Bovin A., Dolgikh A., Dymo A., Kozlov N. Genetically modified legume plants as a basis for studying the signal regulation of symbiosis with nodule bacteria. Ecological genetics. 2023. V. 21. P. 54-55. https://doi.org/10.17816/ecogen568623 (Scopus Q4).
  10. Бовин А.Д., Широбокова С.А, Каракашев Г.В., Долгих Е.А. Синтез инозитолфосфатов в корнях проростков гороха Pisum sativum L. на ранних этапах после инокуляции Rhizobium leguminosarum bv. viciae. Экологическая генетика. 2023. Т. 21, № 1. С. 5-17. doi: 10.17816/ecogen117412 (Scopus Q4).
  11. Bovin A.D., Pavlova O.A., Dolgikh A.V., Leppyanen I.V., Dolgikh E.A. The role of heterotrimeric G-protein beta subunits during nodulation in Medicago truncatula Gaertn and Pisum sativum L. Frontiers in Plant Science. 2022. V. 12: 808573. doi.org/10.3389/fpls.2021.808573 (WoS Q1).
  12. Dolgikh V.V., Senderskiy I.V., Timofeev S.A., Zhuravlyov V.S., Dolgikh A.V., Seliverstova E.V., Ismatullaeva D.A., Mirzakhodjaev B.A. Construction of scFv Antibodies against the Outer Loops of the Microsporidium Nosema bombycis ATP/ADP-Transporters and Selection of the Fragment Efficiently Inhibiting Parasite Growth // Int. J. Mol. Sci. 2022. 23, 15307. https:// doi.org/10.3390/ijms232315307 (WoS Q1).
  13. Velizhanina ME, Galkin AP. Amyloid Properties of the FXR1 Protein Are Conserved in Evolution of Vertebrates // Int. J. Mol. Sci. 2022. 23: 7997. https://doi.org/10.3390/ijms23147997 (WoS Q1).
  14. Rudaya E.S., Kozulina P.Yu., Pavlova O.A., Dolgikh A.V., Ivanova A.N., Dolgikh E.A.  Regulation of the later stages of nodulation stimulated by IPD3/CYCLOPS transcription factor and cytokinin in pea Pisum sativum L. Plants. 2022. V. 11 (1): 56. doi.org/10.3390/plants11010056 (WoS Q1).
  15. Kantsurova E.S., Dolgikh E.A. Features of the regulation of the transcription factor NIN, which determined its participation in the control of nodule organogenesis in legumes plants. Ecological genetics. 2022. V. 20, N 4. P. 20. doi.org/10.17816/ecogen112370 (Scopus). 0.58
  16. Dolgikh E.A., Kantsurova E.S., Dymo A.M. The development of approaches to create new symbiotic systems. Ecological genetics. 2022. V. 20, N 4. P. 9. doi.org/10.17816/ecogen112392 (Scopus Q4).
  17. Leppyanen I.V., Pavlova O.A., Vashurina M.A., Bovin A.D., Dolgikh A.V., Shtark O.Y., Sendersky I. V., Dolgikh V.V., Tikhonovich I.A., Dolgikh E.A. LysM-receptor-like kinase LYK9 of Pisum sativum L. may regulate plant responses to chitooligosaccharides differing in structure. Int. J. Mol. Science. 2021. V. 22 (2): 711. doi.org/10.3390/ijms22020711 (WoS Q1).
  18. Sergeeva AV, Belashova TA, Bondarev SA, Velizhanina ME, Barbitoff YA, Matveenko AG, Valina AA, Simanova AL, Zhouravleva GA, Galkin AP. Direct proof of the amyloid nature of yeast prions [PSI+] and [PIN+] by the method of immunoprecipitation of native fibrils. FEMS Yeast Res. 2021. 21(6): foab046. doi: 10.1093/femsyr/foab046 (WoS, Q1).
  19. Chirinskaite AV, Siniukova VA, Velizhanina ME, Sopova JV, Belashova TA, Zadorsky SP. STXBP1 forms amyloid-like aggregates in rat brain and demonstrates amyloid properties in bacterial expression system. Prion. 2021. 15(1): 29-36. doi: 10.1080/19336896.2021.1883980 (WoS Q2).
  20. Zhukov V.A., Afonin A.M., Akhtemova G.A., Bovin A.D., Dolgikh A.V., Gorshkov A.P., Gribchenko E.S., Ivanova K.A., Kirienko A.N., Kitaeva A.B., Kliukova M.S., Kulaeva O.A., Kusakin P.G., Leppyanen I.V., Pavlova O.A., Romanyuk D.A., Serova T.A., Shtark O.Yu., Sulima A.S., Tsyganova A.V. et al. Transcriptomic response of pea (Pisum sativum L.) plants to inoculation with nodule bacteria, arbuscular­mycorrhizal fungi and PGPB. // FEBS Open Bio. 2021. Т. 11. № S1. С. 390 doi: doi.org/10.1002/2211-5463.13205 (WoS Q1).
  21. Pavlova O.А., Leppyanen I.V., Kustova D.V., Bovin A. D., Dolgikh E. A. Phylogenetic and structural analysis of annexins in pea (Pisum sativum L.) and their role in legume–rhizobial symbiosis development. Vavilov Journal of Genetics and Breeding 2021. V. 25, N 5. P. 502-513 doi: 10.18699/VJ21.057. (WoS, Q4)
  22. Zhukov, V., Zorin, E., Zhernakov, A., Afonin, A., Akhtemova, G., Bovin, A., Dolgikh, A., Gorshkov, A., Gribchenko, E., Ivanova, K., Kirienko, A., Kitaeva, A., Kliukova, M., Kulaeva, O., Kusakin, P., Leppyanen, I., Pavlova, O., Romanyuk, D., Rudaya, E., Serova, T., Shtark, O., Sulima, A., Tsyganova, A., Vasileva, E., Dolgikh, E., Tsyganov, V., & Tikhonovich, I. (2021). Transcriptomic analysis of sym28 and sym29 supernodulating mutants of pea Pisum sativum L.) under complex inoculation with beneficial microorganisms. Biological Communications, 66 (3), 181–197. https:// doi.org/10.21638/spbu03.2021.301 (Scopus Q3).
  23. Рудая Е.С., Долгих Е.А. Получение и анализ композитных растений томата Solanumlycopersicum L., трансформированных генами рецепторов гороха к сигнальным молекулам ризобий. Сельскохозяйственная биология. 2021. Т. 56, № 3, С. 465-474 (Scopus Q4) doi: 10.15389/agrobiology.2021.3.465rus. 0,559
  24. Долгих А.В., Долгих Е.А. Поиск регуляторов, взаимодействующих с транскрипционным фактором BELL1 и необходимых для контроля развития бобово-ризобиального симбиоза. Экологическая генетика. 2021. Т. 19, № 1, С. 37-45 doi: https://doi.org/10.17816/ecogen51489 (Scopus Q4). 0,462
  25. Леппянен И.В., Штарк О.Ю., Павлова О.А., Бовин А.Д., Иванова К.А., Серова Т.С., Долгих Е.А. Анализ эффектов совместной инокуляции грибами арбускулярной микоризы и ризобиями на рост и развитие растений гороха Pisum sativum L. Сельскохозяйственная биология. 2021. Т. 56, № 3, С. 475-486 doi: 10.15389/agrobiology.2021.3.475rus (Scopus Q4) 0,559.
  26. Pavlova O. A., Kirienko A. N., Sendersky I. V., Dolgikh V. V. and Dolgikh E. A. Heterologous synthesis of plant LysM receptor-like kinase K1 in insect cells and binding assay with ligand. 2021. Biotechnology & Biotechnological Equipment, V. 35: sup1, S94. doi: 10.1080/13102818.2020.1871545 (WoS, Q3).
  27. Pavlova O. A., Leppyanen I. V., Senderskiy I. V., Dolgikh V. V., Tikhonovich I. A. and Dolgikh E. A. Synthesis of plant receptor-like kinases involved in interaction with pathogenic and beneficial fungi in heterologous system and their functional analysis. 2021. Biotechnology & Biotechnological Equipment, V. 35: sup1, S92. doi: 10.1080/13102818.2020.1871545 (WoS, Q3).
  28. Dolgikh A.V., Rudaya E.S., Dolgikh E.A. Identification of BELL transcription factors involved in nodule initiation and development in the legumes Pisum sativum and Medicago truncatula. Plants. 2020. V. 9 (12), 1808. doi:10.3390/plants9121808 (WoS Q1).
  29. Dolgikh E.A., Kusakin P.G., Kitaeva A.B., Tsyganova A.V., Kirienko A.N., Leppyanen I.V., Dolgikh A.V., Ilina E.L., Demchenko K.N., Tikhonovich I.A., Tsyganov V. E. Mutational analysis indicates that abnormalities in rhizobial infection and subsequent plant cell and bacteroid differentiation in pea (Pisum sativum) nodules coincide with abnormal cytokinin responses and localization. Annals of Botany, 2020. 125: 905–923.  doi: 10.1093/aob/mcaa022 (WoS Q1).
  30. Smolikova G., Shiroglazova O., Vinogradova G., Leppyanen I., Dinastiya E., Yakovleva O., Dolgikh E., Titova G., Frolov A., Medvedev S. Comparative analysis of the plastid conversion, photochemical activity and chlorophyll degradation in developing embryos of green-seeded and yellow-seeded pea (Pisum sativum) cultivars. Funct Plant Biol. 2020. 47(5): 409-424. doi: 10.1071/FP19270 (WoS Q1).
  31. Afonin A.M., Leppyanen I.V., Kulaeva O.A., Shtark O.Y., Tikhonovich I.A., Dolgikh E.A., Zhukov V.A. A high coverage reference transcriptome assembly of pea (Pisum sativum L.) mycorrhizal roots. Vavilov Journal of Genetics and Breeding 2020. 24(4): 331-339  doi:10.18699/VJ20.625 (WoS Q3).
  32. Bovin A. D., Leppyanen I. V., Pavlova O. A., Dolgikh E. A. The role of heterotrimeric G proteins in the control of symbiosis development in legume plants. BIO Web of Conferences, 2020, 23: 03004 doi.org/10.1051/bioconf/20202303004 (WoS Q4).
  33. Kirienko A.N., Dolgikh E. A. Studying the effect of tissue-specific expression of the K1 gene encoding LysM-receptor-like kinase on the development of symbiosis in peas. BIO Web of Conferences, 2020, 23: 03005 doi.org/10.1051/bioconf/20202303005 (WoS Q4).
  34. Кустова Д.В., Долгих Е.А. Аннексины и их роль в контроле развития симбиозов у растений. Экологическая генетика. 2020.18(3):293-300 doi.org/10.17816/ecogen.183  (Scopus Q4).
  35. Dolgikh A.V., Kirienko A.N., Tikhonovich I.A., Foo E., Dolgikh E.A. The DELLA proteins influence the expression of cytokinin biosynthesis and response genes during nodulation. Front Plant Sci. 2019. 10: 432. doi: 10.3389/fpls.2019.00432 (WoS Q1).
  36. Leppyanen I.V., Kirienko A.N., Dolgikh E.A. Agrobacterium rhizogenes - mediated transformation of Pisum sativum L. roots. PeerJ 2019. 7:e6552 doi.org/10.7717/ peerj.6552 (WoS, Q1).
  37. Kirienko A.N., Vishnevskaya N.A., Kitaeva A.B., Shtark O.Y., Kozyulina P.Y., Thompson R., Dalmais M., Bendahmane A., Tikhonovich I.A., Dolgikh E.A. Structural variations in LysM domains of LysM-RLK PsK1 may result in a different effect on pea-rhizobial symbiosis development. Int. J. Mol. Sci. 2019. V. 20 (7): 1624.  doi:10.3390/ijms20071624 (WoS, Q1).
  38. Бовин А.Д., Долгих Е.А. Роль гетеротримерных G-белков в сигнальной регуляции у растений // Экологическая генетика. 2019.  Т. 17, № 2.  С. 43–54 doi.org/10.17816/ecogen17243-54 (Scopus Q4). 
  39. Долгих А. В., Долгих Е. А. Роль универсальных регуляторов роста и развития растений DELLA-белков в контроле симбиозов // Экологическая генетика.  2019. Т. 17,  № 1.  С. 33–41 (Scopus Q4).

 

Patents:

  1. Dolgikh E.A., Kiriyenko A.N. A recombinant vector for the synthesis of receptor kinase K1 in plant cells, which controls the development of symbiosis with nodule bacteria, and a strain for replication of the vector. Patent No. 2738735 dated 12.12.2018. The patent was registered on 12/16/2020.
  2. Dolgikh E.A., Dolgikh V.V., Leppyanen I.V., Varlamov V.P., Lopatin S.A., Tikhonovich I.A. Method of enzymatic production of penta-N-acetylchytopentaose // Patent No. 2460800 dated 08/04/2010. The patent was registered on 09/10/2012.
  3. Dolgikh E.A., Dolgikh V.V., Leppyanen I.V., Varlamov V.P., Lopatin S.A., Tikhonovich I.A. Method of enzymatic production of penta-N-acetylchitopentaose and hexa-N-acetylchitohexaose. Patent No. 2517620 dated 08/04/2010. The patent was registered on 04/01/2014.

 

Dissertations:

Bovin A.D. "The role of heterotrimeric G-proteins in the regulation of the development of legume-rhizobial symbiosis in alfalfa M. truncatula Gaertn. and peas P. sativum L." specialty 03.02.03 microbiology, 2022

Kanzurova Elizaveta Stepanovna “Structural organization and regulation features of the transcription factor NIN, which determined its participation in the control of the development of rhizobial symbiosis"
specialties 1.5.11. – Microbiology, 1.5.21 Physiology and biochemistry of plants

Subscribe to the newsletter of FSBCI ARRIAM

Что будем искать?