FSBSI ARRIAM
Federal State Budget Scientific Institution
All-Russia Research Institute for Agricultural Microbiology
All-Russia Research Institute for Agricultural Microbiology
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For many mutant pea lines with nodule development disorders, the genes affected by the mutations are not yet known. The laboratory recently obtained RNA sequencing data from F2 plants for genetic mapping of the symbiotic genes Sym23 and Sym24 using a modified Mapping-by-sequencing technique (the technique is described in detail in Zhernakov et al., 2019). RNA sequencing data for mutant plant nodules were also obtained. In the course of the work, it will be necessary to conduct a detailed bioinformatics analysis of the available data to identify candidate genes and to describe in detail the gene expression profiles in mutant nodules compared to the nodules of the original "wild type" line. As a result, new fundamental knowledge will be obtained about the pea genetic system that controls the development and functioning of nitrogen-fixing nodules.
Within the framework of this work, it is possible to perform further tasks on the genetic transformation of mutant pea lines with constructs containing candidate genes for complementation of mutations. Further development of the directions of work will depend on the functions of the identified genes.
Scientific supervisor: Dr. O.A. Kulaeva.
