FSBSI ARRIAM
Federal State Budget Scientific Institution
All-Russia Research Institute for Agricultural Microbiology
All-Russia Research Institute for Agricultural Microbiology
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Pea forms symbioses with various soil microorganisms – nodule bacteria, arbuscular mycorrhiza fungi and growth-promoting bacteria. Despite the differences in these symbioses, a number of common regulatory and structural genes are involved in their development. The question remains open as to whether the expression of plant genes differs under conditions of single and multiple inoculations (whether the influence of different symbioses on each other is observed and what it is – mutual suppression or a synergistic effect). To answer this question, a large-scale pot experiment was conducted, in which the transcriptome of the roots of two pea varieties inoculated with different combinations of microorganisms (nodule bacteria, arbuscular mycorrhiza fungi and growth-promoting bacteria) was sequenced. The proposed study plans to analyze differential gene expression using modern bioinformatics approaches, characterize the response of pea plants to multiple and single inoculation, and identify molecular markers of effective symbiosis development suitable for use in the breeding process. RNA sequencing data according to the MACE protocol have already been obtained and are waiting for a student who is ready to process them and who has basic skills in R. The results of a similar, although smaller-scale, study can be found here: Kuzmina et al., 2025.
Scientific supervisor: Dr. V.A. Zhukov.
