FSBSI ARRIAM
Federal State Budget Scientific Institution
All-Russia Research Institute for Agricultural Microbiology
All-Russia Research Institute for Agricultural Microbiology
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The use of biopreparations containing strains of nodule bacteria for peas is complicated by the fact that there are strains in the soil that are also capable of establishing symbiosis with peas, but are less effective in fixing nitrogen than the strains from the preparation. Competition between native and introduced strains can lead to formation of nodules mostly by ineffective strains, as a result of which the positive effect of the preparation may not occur. It is necessary to create a simple and convenient test system that allows tracking what proportion of nodules on plant roots is formed by the strain from the biopreparation, and what proportion is formed by native strains. Based on the available data on the sequenced genome of one of the production strains, it is necessary to select unique sequences and create markers for analysis using qPCR. This analysis will allow determining the percentage of nodules formed by native microflora and the production strain, using dried pea roots from field experiments. The work will also require analyzing previously obtained data on nodule metagenomes from various field experiments, which can be compared with the results of the analysis of the test system being developed. This system will be used in the future to select the source material for breeding new pea varieties that specifically interact with strains from the biopreparation.
Scientific supervisor: Dr. V.A. Zhukov, Dr. A.S. Sulima.
